An artificial pathway to 3, 4-dihydroxybenzoic acid allows generation of new aminocoumarin antibiotic recognized by catechol transporters of E. coli
S Alt, N Burkard, A Kulik, S Grond, L Heide
Index: Alt, Silke; Burkard, Nadja; Kulik, Andreas; Grond, Stephanie; Heide, Lutz Chemistry and Biology, 2011 , vol. 18, # 3 p. 304 - 313
Full Text: HTML
Citation Number: 17
Abstract
An artificial operon was synthesized, consisting of the genes for chorismate pyruvate-lyase of E. coli and for 4-hydroxybenzoate 3-hydroxylase of Corynebacterium cyclohexanicum. This operon, directing the biosynthesis of 3, 4-dihdroxybenzoate, was expressed in the heterologous expression host Streptomyces coelicolor M512, together with a modified biosynthetic gene cluster for the aminocoumarin antibiotic clorobiocin. The resulting strain ...
Related Articles:
[Le Bras, Gaelle; Radanyi, Christine; Peyrat, Jean-Francois; Brion, Jean-Daniel; Alami, Mouad; Marsaud, Veronique; Stella, Barbara; Renoir, Jack-Michel Journal of Medicinal Chemistry, 2007 , vol. 50, # 24 p. 6189 - 6200]