Carbohydrate research

The use of fluoro-and deoxy-substrate analogs to examine binding specificity and catalysis in the enzymes of the sorbitol pathway

ME Scott, RE Viola

Index: Scott, Mary Ellen; Viola, Ronald E. Carbohydrate Research, 1998 , vol. 313, # 3-4 p. 247 - 253

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Citation Number: 17

Abstract

The carbohydrate specificity of the two enzymes that catalyze the metabolic interconversions in the sorbitol pathway, aldose reductase and sorbitol dehydrogenase, has been examined through the use of fluoro-and deoxy-substrate analogs. Hydrogen bonding has been shown to be the primary mode of interaction by which these enzymes specifically recognize and bind their respective polyol substrates. Aldose reductase has broad substrate specificity, ...