On the use of nonfluorescent dye labeled ligands in FRET-based receptor binding studies

C Tahtaoui, F Guillier, P Klotz, JL Galzi…

Index: Tahtaoui, Chouaib; Guillier, Fabrice; Klotz, Philippe; Galzi, Jean-Luc; Hibert, Marcel; Ilien, Brigitte Journal of Medicinal Chemistry, 2005 , vol. 48, # 24 p. 7847 - 7859

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Citation Number: 27

Abstract

The efficiency of fluorescence resonance energy transfer (FRET) is dependent upon donor- acceptor proximity and spectral overlap, whether the acceptor partner is fluorescent or not. We report here on the design, synthesis, and characterization of two novel pirenzepine derivatives that were coupled to patent blue VF and pinacyanol dyes. These nonfluorescent compounds, when added to cells stably expressing enhanced green fluorescent protein ( ...