Carbohydrate research

An α-l-fucosidase from Penicillium multicolor as a candidate enzyme for the synthesis of α (1⃗3)-linked fucosyl oligosaccharides by transglycosylation

K Ajisaka, H Fujimoto, M Miyasato

Index: Ajisaka, Katsumi; Fujimoto, Hiroshi; Miyasato, Mariko Carbohydrate Research, 1998 , vol. 309, # 1 p. 125 - 129

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Citation Number: 44

Abstract

A new α-l-fucosidase was partially purified from the culture broth of Penicillium multicolor, which was available commercially as a freeze dried powder by the name of Lactase-P™. This enzyme catalysed the transglycosylation of fucose residue of p-nitrophenyl-α-l- fucopyranoside to give α-l-Fuc-(1⃗3)-d-Glc or α-l-Fuc-(1⃗3)-d-GlcNAc regioselectively. This enzyme was more stable in the organic co-solvents than the α-fucosidase from Aspergillus ...