Antibody??Catalyzed Removal of the p??Nitrobenzyl Ester Protecting Group: The Molecular Basis of Broad Substrate Specificity

S Kurihara, T Tsumuraya, K Suzuki…

Index: Kurihara, Shinwa; Tsumuraya, Takeshi; Suzuki, Kayo; Kuroda, Masataka; Liu, Lidong; Takaoka, Yumiko; Fujii, Ikuo Chemistry - A European Journal, 2000 , vol. 6, # 9 p. 1656 - 1662

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Citation Number: 20

Abstract

Abstract Antibody catalysts for the removal of the p-nitrobenzyl ester protecting group have been generated to accommodate a broad range of substrates. Antibody 7B9, which was elicited against p-nitrobenzyl phosphonate 1, catalyzed the hydrolyses of p-nitrobenzyl monoesters of nonsubstituted, and β-and γ-substituted glutaric acids with almost identical K m and k cat values. In addition, 7B9 displayed substrate tolerance towards the α- ...