Practical preparation of lacto-N-biose I, a candidate for the bifidus factor in human milk

M Nishimoto, M Kitaoka

Index: Nishimoto, Mamoru; Kitaoka, Motomitsu Bioscience, Biotechnology and Biochemistry, 2007 , vol. 71, # 8 p. 2101 - 2104

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Citation Number: 91

Abstract

A one-pot enzymatic reaction to produce lacto-N-biose I (LNB), which is supposed to represent the bifidus factor in human milk oligosaccharides, was demonstrated. Approximately 500 mm of LNB was generated in 10-liter of reaction mixture initially containing 660 mm of sucrose and 600 mM of GlcNAc by the concurrent actions of four enzymes, sucrose phosphorylase, UDP-glucose—hexose-1-phospate uridylyltransferase, ...