Tailoring DNA structure to increase target hybridization kinetics on surfaces

AE Prigodich, OS Lee, WL Daniel…

Index: Lee, Jun Hee; Zhang, Yandong; Danishefsky, Samuel J. Journal of the American Chemical Society, 2010 , vol. 132, # 41 p. 14330 - 14333

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Citation Number: 55

Abstract

We report a method for increasing the rate of target hybridization on DNA-functionalized surfaces using a short internal complement DNA (sicDNA) strand. The sicDNA causes up to a 5-fold increase in association rate by inducing a conformational change that extends the DNA away from the surface, making it more available to bind target nucleic acids. The sicDNA-induced kinetic enhancement is a general phenomenon that occurred with all ...