Synthesis and Some Biochemical Properties of a Novel 5, 6, 7, 8??Tetrahydropyrimido [4, 5??c] pyridazine Nucleoside

…, SA Salisbury, MG McDougall, C Neagu…

Index: Loakes, David; Brown, Daniel M.; Salisbury, Stephen A.; McDougall, Mark G.; Neagu, Constantin; Nampalli, Satyam; Kumar, Shiv Helvetica Chimica Acta, 2003 , vol. 86, # 4 p. 1193 - 1204

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Citation Number: 23

Abstract

Abstract A novel nucleoside analogue is described based on the pyridazine ring system. The nucleoside was successfully incorporated into DNA via both its phosphoramidite and 5′-triphosphate derivatives. Enzymatically, the analogue behaves essentially as thymidine: it is a good substrate for the DNA polymerases Taq and exonuclease-free Klenow fragment, leading to full-length products when present in either the primer or template strands. In ...