Synthesis of arabinitol 1-phosphate and its use for characterization of arabinitol–phosphate dehydrogenase

…, AA Kulminskaya, EV Eneyskaya, KA Shabalin…

Index: Soroka, Nikolai V.; Kulminskaya, Anna A.; Eneyskaya, Elena V.; Shabalin, Konstantin A.; Uffimtcev, Andrei V.; Povelainen, Mira; Miasnikov, Andrei N.; Neustroev, Kirill N. Carbohydrate Research, 2005 , vol. 340, # 4 p. 539 - 546

Full Text: HTML

Citation Number: 3

Abstract

d-Arabinitol 1-phosphate (Ara-ol1-P), a substrate for d-arabinitol–phosphate dehydrogenase (APDH), was chemically synthesized from d-arabinonic acid in five steps (O-acetylation, chlorination, reduction, phosphorylation, and de-O-acetylation). Ara-ol1-P was used as a substrate for the characterization of APDH from Bacillus halodurans. APDH converts Ara-ol1- P to xylulose 5-phosphate in the oxidative reaction; both NAD+ and NADP+ were ...