Synthesis of a new bifunctionalised fluorescent label and physical properties of the bound form on model peptide of troponin C

…, S Iyoshi, M Taki, Y Maeda, Y Yamamoto

Index: Hirayama, Tasuku; Iyoshi, Shohei; Taki, Masayasu; Maeda, Yuichiro; Yamamoto, Yukio Organic and Biomolecular Chemistry, 2007 , vol. 5, # 13 p. 2040 - 2045

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Citation Number: 11

Abstract

A new bifunctional fluorescent label, BRos, was synthesised in order to monitor protein dynamics using fluorescence microscopy, and the photophysical properties were compared with those of bifunctionalised rhodamine, BRho. In a labelling experiment with a model peptide of troponin C, which regulates muscle contraction and relaxation, it was found that BRos was bound to the peptide through two linkages and provided a homogeneous ...