Molecular tripods showing fluorescence enhancement upon binding to streptavidin

…, HY Yoon, J Park, OH Kwon, DJ Jang, JI Hong

Index: Tae, Woo Kim; Hey, Young Yoon; Park, Jung-Hyun; Kwon, Oh-Hoon; Jang, Du-Jeon; Hong, Jong-In Organic Letters, 2005 , vol. 7, # 1 p. 111 - 114

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Citation Number: 27

Abstract

We introduce a new approach to biotin-streptavidin assays based on a molecular tripod which consists of biotin, a fluorophore, and a quencher. The interaction between streptavidin and molecular tripods perturbs the ground-state quencher-fluorophore dimeric conformation in the absence of streptavidin and diminishes the intrinsic self-quenching of a quencher- fluorophore pair. The emission intensity of the molecular tripods plus streptavidin is 3.5- ...