A new class of phospholipase A2 substrates: kinetics of the phospholipase A2 catalyzed hydrolysis of 3-(acyloxy)-4-nitrobenzoic acids

W Cho, MA Markowitz, FJ Kezdy

Index: Cho, Wonhwa; Markowitz, Michael A.; Kezdy, Ferenc J. Journal of the American Chemical Society, 1988 , vol. 110, # 15 p. 5166 - 5171

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Citation Number: 30

Abstract

Abstract: 3-(Acyloxy)-4-nitrobenzoic acids were synthesized with acyl groups ranging from butyryl to dodecanoyl. All these compounds yielded monomeric solutions in water with 1.6%(v/v) acetonitrile in the neutral pH range, and they were hydrolyzed by catalytic amounts of phospholipases A, from a variety of sources as shown by the spectral change at 425 nm due to the appearance of nitrophenolate ion. Most of the kinetic studies were ...