The α-Chymotrypsin-catalyzed Hydrolysis of Acetyl-, Chloroacetyl-and Benzoyl-L-valine Methyl Ester1
TH Applewhite, H Waite, C Niemann
Index: Applewhite et al. Journal of the American Chemical Society, 1958 , vol. 80, p. 1465,1468
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Citation Number: 35
Abstract
A search for neutral ester type specific substrates of a-chymotrypsin usable with enzyme concentrations of the order of 10-5. I1 has resulted in the selection of several acylated-L- valine methyl esters. The constants KS and k, have been evaluated for the a-chymotrypsin- catalyzed hydrolysis of acetyl-, chloroacetyl-and benzoyl-L-valine methyl ester in aqueous solutions at 25.0'and pH 7.90+ 0.01 and 0.1. lI in sodiuni chloride and for the latter specific ...
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