A mechanism-based fluorescence transfer assay for examining ketosynthase selectivity

…, LS Borketey, TY Lin, NA Schnarr

Index: Prasad, Gitanjeli; Borketey, Lawrence S.; Lin, Tsung-Yi; Schnarr, Nathan A. Organic and Biomolecular Chemistry, 2012 , vol. 10, # 33 p. 6717 - 6723

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Citation Number: 5

Abstract

Since their discovery, polyketide synthases have received massive attention from researchers hoping to harness their potential as a platform for generating new and improved therapeutics. Despite significant strides toward this end, inherent specificities within the enzymes responsible for polyketide production have severely limited these efforts. We have developed a mechanism-based, fluorescence transfer assay for a key enzyme component ...