Biochemistry

Novel heterocyclic analogues of firefly luciferin

CC Woodroofe, PL Meisenheimer, DH Klaubert…

Index: Woodroofe, Carolyn C.; Meisenheimer, Poncho L.; Klaubert, Dieter H.; Kovic, Yumi; Rosenberg, Justin C.; Behney, Curran E.; Southworth, Tara L.; Branchini, Bruce R. Biochemistry, 2012 , vol. 51, # 49 p. 9807 - 9813

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Citation Number: 24

Abstract

Five novel firefly luciferin analogues in which the benzothiazole ring system of the natural substrate was replaced with benzimidazole, benzofuran, benzothiophene, benzoxazole, and indole were synthesized. The fluorescence, bioluminescence, and kinetic properties of the compounds were evaluated with recombinant Photinus pyralis wild type luciferase. With the exception of indole, all of the substrates containing heterocycle substitutions produced ...

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