Bioorganic & medicinal chemistry letters

A novel biotinylated diazirinyl ceramide analogue for photoaffinity labeling

M Hashimoto, Y Hatanaka

Index: Hashimoto, Makoto; Hatanaka, Yasumaru Bioorganic and Medicinal Chemistry Letters, 2008 , vol. 18, # 2 p. 650 - 652

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Citation Number: 6

Abstract

A novel photoreactive ceramide analogue, which contains (3-trifluoromethyl) phenyldiazirinyl lipid and biotinylated sphingosine, was synthesized. The probe was recognized as an antigen by anti-ceramide antibody and as a substrate for sphingolipid ceramide N-deacylase. These results indicate that the probe may be useful as a photoaffinity- biotinylating agent in sphingolipid studies.