Fluorous affinity purification of oligonucleotides

WH Pearson, DA Berry, P Stoy, KY Jung…

Index: Pearson, William H.; Berry, David A.; Stoy, Patrick; Jung, Kee-Yong; Sercel, Anthony D. Journal of Organic Chemistry, 2005 , vol. 70, # 18 p. 7114 - 7122

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Citation Number: 74

Abstract

Nucleoside phosphoramidites bearing a fluorous dimethoxytrityl (FDMT) group were used to synthesize fluorous-tagged oligonucleotides, which were subjected to solid-phase extraction using a pH-stable fluorinated adsorbent. On-column detritylation afforded the purified oligonucleotides. The fluorous affinity purification method offers one-pass loading without ammonia removal, high selectivity for the removal of failure sequences, high recoveries ( ...