Study of the interaction between 2, 5-di-[2-(4-hydroxy-phenyl) ethylene]-terephthalonitril and bovine serum albumin by fluorescence spectroscopy

LN Zhang, FY Wu, AH Liu

Index: Zhang, Li-Na; Wu, Fang-Ying; Liu, Ai-Hong Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 2011 , vol. 79, # 1 p. 97 - 103

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Citation Number: 50

Abstract

A new compound, 2, 5-di-[2-(4-hydroxy-phenyl) ethylene]-terephthalonitrile (DHPEPN), was synthesized. The interaction between bovine serum albumin (BSA) and DHPEPN in Tris– HCl buffer solution (pH 7.4) was investigated using fluorescence and UV–vis absorption spectroscopy. The mechanism of BSA fluorescence quenched by DHPEPN is discussed according to the Stern–Volmer equation. The binding constant and the thermodynamic ...