pH-rate profiles of L-arabinitol 4-dehydrogenase from Hypocrea jecorina and its application in L-xylulose production

MK Tiwari, RK Singh, H Gao, T Kim, S Chang…

Index: Tiwari, Manish Kumar; Singh, Raushan Kumar; Gao, Hui; Kim, Taesu; Chang, Suhwan; Kim, Han S.; Lee, Jung-Kul Bioorganic and Medicinal Chemistry Letters, 2014 , vol. 24, # 1 p. 173 - 176

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Citation Number: 9

Abstract

Abstract l-Arabinitol 4-dehydrogenase (LAD) from Hypocrea jecorina (HjLAD) was cloned and overexpressed in Escherichia coli BL21 (DE3). The kinetics of l-arabinitol oxidation by NAD+, catalyzed by HjLAD, was studied within the pH range of 7.0–9.5 at 25 C. The turnover number (k cat) and the catalytic efficiency (k cat/K m) were 4200 min− 1 and 290 mM− 1 min− 1, respectively. HjLAD showed the highest turnover number and catalytic efficiency ...