Synthesis and Photochemistry of a New Class of Photocleavable Protein Cross??linking Reagents

L Milanesi, GD Reid, GS Beddard…

Index: Milanesi, Lilia; Reid, Gavin D.; Beddard, Godfrey S.; Hunter, Christopher A.; Waltho, Jonathan P. Chemistry - A European Journal, 2004 , vol. 10, # 7 p. 1705 - 1710

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Citation Number: 12

Abstract

Abstract A new series of photocleavable protein cross-linking reagents based on bis (maleimide) derivatives of diaryl disulfides have been synthesised. They have been functionalised with cysteine and transient absorption spectra for the photolysis reaction have been recorded by using the pump-probe technique with a time resolution of 100 femtoseconds. Photolysis of the disulfide bond yields the corresponding thiyl radicals in ...