Guanine specific DNA cleavage by photoirradiation of dibenzoyldiazomethane-oligonucleotide conjugates

K Nakatani, J Shirai, S Sando…

Index: Nakatani, Kazuhiko; Shirai, Junya; Sando, Shinsuke; Saito, Isao Journal of the American Chemical Society, 1997 , vol. 119, # 33 p. 7626 - 7635

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Citation Number: 28

Abstract

Photoirradiation of dibenzoyldiazomethane (DBDM) produced highly electrophilic benzoylketene via Wolff rearrangement. DBDM derivative possessing an aminoalkyl side chain induced a DNA cleavage selectively at guanine (G) residues upon photoirradiation and subsequent piperidine treatment. In order to devise photochemical DNA cleavers that can specifically alkylate a guanine residue proximal to the target sequence of long DNA ...