Biocatalytic and structural properties of a highly engineered halohydrin dehalogenase

M Schallmey, RJ Floor, B Hauer, M Breuer…

Index: Schallmey, Marcus; Floor, Robert J.; Hauer, Bernhard; Breuer, Michael; Jekel, Peter A.; Wijma, Hein J.; Dijkstra, Bauke W.; Janssen, Dick B. ChemBioChem, 2013 , vol. 14, # 7 p. 870 - 881

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Citation Number: 24

Abstract

Abstract Two highly engineered halohydrin dehalogenase variants were characterized in terms of their performance in dehalogenation and epoxide cyanolysis reactions. Both enzyme variants outperformed the wild-type enzyme in the cyanolysis of ethyl (S)-3, 4- epoxybutyrate, a conversion yielding ethyl (R)-4-cyano-3-hydroxybutyrate, an important chiral building block for statin synthesis. One of the enzyme variants, HheC2360, ...