Catalytic antibodies with acyl-transfer capabilities: mechanistic and kinetic investigations

…, DM Schloeder, MI Weinhouse, RA Lerner…

Index: Janda, Kim D.; Ashley, Jon A.; Jones, Teresa M.; McLeod, Donald A.; Schloeder, Diane M.; Weinhouse, Michael I.; Lerner, Richard A.; Gibbs, Richard A.; Benkovic, Patricia A.; Hilhorst, Riet; Benkovic, Stephen J. Journal of the American Chemical Society, 1991 ,  vol. 113,  # 1  p. 291 - 297

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Citation Number: 46

Abstract

Abstract: Antibodies have been shown to catalyze acyl-transfer reactions. The processes by which they perform such tasks have often been postulated but largely remain unknown. An extended study is presented on three different monoclonal antibodies that catalyze the hydrolysis of an alkyl ester and an aryl amide bond. Antibodies 2H6 and 21H3 catalyze the hydrolysis of an unactivated benzyl ester and show exquisite specificity for substrates with ...