Pyrene-labeled oligodeoxynucleotide probe for detecting base insertion by excimer fluorescence emission

A Okamoto, T Ichiba, I Saito

Index: Okamoto, Akimitsu; Kanatani, Keiichiro; Saito, Isao Journal of the American Chemical Society, 2004 , vol. 126, # 15 p. 4820 - 4827

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Citation Number: 148

Abstract

Novel ODN probes for insertion polymorphism detection have been devised. The ODN probes labeled with two pyrene chromophores emit strong excimer fluorescence when hybridized with DNA containing one inserted base. This method is useful for the determination of the presence/absence of insertion polymorphisms located at a specific site on the target DNA.