Bioscience, biotechnology, and biochemistry

Purification and Characterization of a (R)-1-Phenyl-1, 3-propanediol-producing Enzyme from Trichosporon fermentans AJ-5152 and Enzymatic (R)-1-Phenyl-1, 3- …

I Kira, N Onishi

Index: Kira, Ikuo; Onishi, Norimasa Bioscience, Biotechnology and Biochemistry, 2009 , vol. 73, # 7 p. 1640 - 1646

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Citation Number: 9

Abstract

An (R)-1-phenyl-1, 3-propanediol-producing enzyme was purified from Trichosporon fermentans AJ-5152. It was NADPH-dependent and converted 3-hydroxy-1-phenylpropane- 1-one (HPPO) to (R)-1-phenyl-1, 3-propanediol [(R)-PPD] with anti-Prelog's specificity. It showed maximum activity at pH 7.0 and 40° C. Its K m and V max values toward HPPO were 20.1 mm and 3.4 μmol min− 1 mg protein− 1 respectively. The relative molecular weight of ...