Effect of prolonged exposure to organic solvents on the active site environment of subtilisin Carlsberg

…, A Ferrer, K Griebenow, F Secundo, GL Barletta

Index: Bansal, Vibha; Delgado, Yamixa; Fasoli, Ezio; Ferrer, Amaris; Griebenow, Kai; Secundo, Francesco; Barletta, Gabriel L. Journal of Molecular Catalysis B: Enzymatic, 2010 , vol. 64, # 1-2 p. 38 - 44

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Citation Number: 9

Abstract

The potential of enzyme catalysis as a tool for organic synthesis is nowadays indisputable, as is the fact that organic solvents affect an enzyme's activity, selectivity and stability. Moreover, it was recently realized that an enzyme's initial activity is substantially decreased after prolonged exposure to organic media, an effect that further hampers their potential as catalysts for organic synthesis. Regrettably, the mechanistic reasons for these effects are ...