Screening for new hydroxynitrilases from plants

…, T Ueatrongchit, A H-KITTIKUN, T Ohmiya

Index: Asano, Yasuhisa; Tamura, Ken'ichirou; Doi, Nobutaka; Ueatrongchit, Techawaree; H-Kittikun, Aran; Ohmiya, Tohru Bioscience, Biotechnology and Biochemistry, 2005 , vol. 69, # 12 p. 2349 - 2357

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Citation Number: 57

Abstract

We established a simple HPLC method to determine the activity and stereochemistry of the chiral mandelonitrile synthesized from benzaldehyde and cyanide, and applied it to screen for hydroxynitrile lyase (HNL) activity of plant origin. A total of 163 species of plants among 74 families were examined for (R)-and (S)-HNL activities using the method. We discovered that homogenate of leaves of Baliospermum montanum shows (S)-HNL activity, while ...