Organic letters

Stereochemical Analysis of Isopentenyl Diphosphate Isomerase Type II from Staphylococcus a ureus Using Chemically Synthesized (S)-and (R)-[2-2H] Isopentenyl …

C Kao, W Kittleman, H Zhang, H Seto, H Liu

Index: Kao, Chai-Lin; Kittleman, William; Zhang, Hua; Seto, Haruo; Liu, Hung-Wen Organic Letters, 2005 , vol. 7, # 25 p. 5677 - 5680

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Citation Number: 19

Abstract

To study the catalysis of isopentenyl diphosphate (IPP) isomerase type II from Staphylococcus aureus, which is a flavoprotein catalyzing the interconversion of IPP and dimethylallyl diphosphate, we have chemically synthesized (S)-and (R)-[2-2H] IPP and carried out stereochemical analysis of the reaction. Our results show that the C-2 deprotonation of IPP by this enzyme is pro-R stereospecific, suggesting a similar ...