Assay of labile estrogen o-quinones, potent carcinogenic molecular species, by high performance liquid chromatography–electrospray ionization tandem mass …

K Yamashita, A Masuda, Y Hoshino, S Komatsu…

Index: Yamashita, Kouwa; Masuda, Akina; Hoshino, Yuka; Komatsu, Sachiko; Numazawa, Mitsuteru Journal of Steroid Biochemistry and Molecular Biology, 2010 , vol. 119, # 3-5 p. 141 - 148

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Citation Number: 8

Abstract

A sensitive and selective assay method for labile estrogen o-quinones, estrone (E1)-2, 3- quinone (Q), E1-3, 4-Q, estradiol (E2)-2, 3-Q and E2-3, 4-Q, based on the use of phenazine (Phz) derivatization with o-phenylenediamine and high performance liquid chromatography– electrospray ionization tandem mass spectrometry (HPLC–ESI-MS/MS) was described. The Phz derivatives of four estrogen o-quinones were purified by solid phase extraction and ...