Analytical chemistry

Optically trapping confocal Raman microscopy of individual lipid vesicles: kinetics of phospholipase A2-catalyzed hydrolysis of phospholipids in the membrane bilayer

DP Cherney, GA Myers, RA Horton…

Index: Cherney, Daniel P.; Myers, Grant A.; Horton, Robert A.; Harris, Joel M. Analytical Chemistry, 2006 , vol. 78, # 19 p. 6928 - 6935

Full Text: HTML

Citation Number: 26

Abstract

Phospholipase A2 (PLA2)-catalyzed hydrolysis at the sn-2 position of 1, 2-dimyristoyl-sn- glycero-3-phosphocholine in optically trapped liposomes is monitored in situ using confocal Raman microscopy. Individual optically trapped liposomes (0.6 μm in diameter) are exposed to PLA2 isolated from cobra (Naja naja naja) venom at varying enzyme concentrations. The relative Raman scattering intensities of CC stretching vibrations from the trans and gauche ...