Design and synthesis of sulfur??35 agents and their applications for protein labeling

…, P McNamara, D Koharski, D Hesk…

Index: Ren, Sumei; McNamara, Paul; Koharski, David; Hesk, David; Borges, Scott Journal of Labelled Compounds and Radiopharmaceuticals, 2009 , vol. 52, # 8 p. 316 - 323

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Abstract

Abstract Two new 35 S reagents were developed to radiolabel proteins. The first reagent, N- succinimidyl-4-(methane [35 S] sulfonylamino-methyl)-benzoate (SMSB), acylates the ε- amino group of lysine residues in proteins. The second reagent, 4-(methane [35 S] sulfonylamino-methyl)-phenylpropylaldehyde (MSAPPA), labels lysine residues via reductive alkylation. Comparing the two methods, the reductive alkylation method labeled ...