A cleavable amino-thiol linker for reversible linking of amines to DNA

JW Højfeldt, P Blakskjær, KV Gothelf

Index: Hojfeldt, Jonas W.; Blakskjaer, Peter; Gothelf, Kurt V. Journal of Organic Chemistry, 2006 , vol. 71, # 25 p. 9556 - 9559

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Citation Number: 14

Abstract

A cleavable heterobifunctional cross-linker for the reversible conjugation of amines to thiol- modified DNA has been developed and tested. The succinimidyl 2-(vinylsulfonyl) ethyl carbonate (SVEC) was prepared in three steps and tested for its ability to react with amines and thiols. The linker was efficient for binding leucine to a thiol-modified DNA sequence and for releasing the amino acid at pH 11.8.