Carbohydrate research

4-Methylumbelliferyl 2-acetamido-2-deoxy-α-d-glucopyranoside, a fluorogenic substrate for N-acetyl-α-D-glucos-aminidase

P Chow, B Weissmann

Index: Chow, Philip; Weissmann, Bernard Carbohydrate Research, 1981 , vol. 96, p. 87 - 94

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Citation Number: 21

Abstract

Abstract Condensation of dimeric 3, 4, 6-tri-O-acetyl-2-deoxy-2-nitroso-α-d-glucopyranosyl chloride with 4-methylumbelliferone gave crystalline 4-methylumbelliferyl 3, 4, 6-tri-O-acetyl- 2-deoxy-2-oximino-α-d-arabino-hexopyranoside. Acetylation of this adduct, reduction of the resulting crude O-acetyloxime with borane in oxolane, and acetylation gave the 3, 4, 6-tri-O- acetyl derivative of 4-methylumbelliferyl 2-acetamido-2-deoxy-α-d-glucopyranoside (1). A ...