Quantification of binding of some thiol-reactive clenbuterol analogues to bovine serum albumin by electron paramagnetic resonance spectroscopy

A Preželj, J Štrancar, F Gubenšek, S Pečar…

Index: Prezelj, Andrej; Pecar Pharmazie, 2002 , vol. 57, # 1 p. 70 - 71

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Citation Number: 3

Abstract

The only free thiol group of bovine serum albumin (BSA) was coupled in a high yield with some novel thiol-reactive clenbuterol analogues. The unreacted cysteines were probed with maleimide spin label to determine the yield of the coupling reaction. A novel approach to