Synthesis of hydrophilic and flexible linkers for peptide derivatization in solid phase

A Song, X Wang, J Zhang, J Mařı́k, CB Lebrilla…

Index: Song, Aimin; Wang, Xiaobing; Zhang, Jinhua; Marik, Jan; Lebrilla, Carlito B.; Lam, Kit S. Bioorganic and Medicinal Chemistry Letters, 2004 , vol. 14, # 1 p. 161 - 165

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Citation Number: 55

Abstract

Four N-Fmoc protected polyoxyethylene-based amino acid type linkers were designed and synthesized for peptide derivatization in solid phase. Three of them were obtained in a crystalline form. The crystallized linkers can be stored at 4° C for 2 years without significant decomposition. Protocols for biotinylation and fluorescent labeling of peptides in solid phase were developed. The linkers also provide good ionization ability for single-bead mass ...