Agricultural and biological chemistry

Preparation and characterization of a cleavable photoactivable heterobifunctional fluorescent reagent.

K Muramoto, H Kamiya

Index: Muramoto, Koji; Kamiya, Hisao Agricultural and Biological Chemistry, 1988 , vol. 52, # 2 p. 547 - 554

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Citation Number: 7

Abstract

A cleavable photoactivable heterobifunctional reagent, the N-hydroxysuccinimide ester of 1- azido-5-naphthalene sulfonyl S-carboxymethylthiocysteamine (NHS-ANS-CTC), was synthesized and characterized. The reagent was applicable to the group-directed modification of protein ligands, such as an invertebrate lectin and a trypsin inhibitor. The modified ligands bound to rabbit erythrocyte ghosts and trypsin, respectively. Upon ...