Multivalent Design of Apoptosis??Inducing Bid??BH3 Peptide–Oligosaccharides Boosts the Intracellular Activity at Identical Overall Peptide Concentrations

…, M Beyermann, R Brock, J Rademann

Index: Richter, Martin; Chakrabarti, Alokta; Ruttekolk, Ivo R.; Wiesner, Burkhard; Beyermann, Michael; Brock, Roland; Rademann, Joerg Chemistry - A European Journal, 2012 , vol. 18, # 52 p. 16708 - 16715

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Citation Number: 17

Abstract

Abstract Multivalent peptide–oligosaccharide conjugates were prepared and used to investigate the multivalency effect concerning the activity of Bid-BH3 peptides in live cells. Dextran oligosaccharides were carboxyethylated selectively in the 2-position of the carbohydrate units and activated for the ligation of N-terminally cysteinylated peptides. Ligation through maleimide coupling was found to be superior to the native chemical ...