Carbohydrate Research

p-Aminophenyl β-cellobioside as an affinity ligand for exo-type cellulases

K Sangseethong, MH Penner

Index: Sangseethong, Kunruedee; Penner, Michael H. Carbohydrate Research, 1998 , vol. 314, # 3-4 p. 245 - 250

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Citation Number: 14

Abstract

p-Aminophenyl β-cellobioside (PAPC) is shown to be an effective affinity ligand for the chromatographic fractionation of cellobiohydrolases (CBHs). A crude cellulase preparation from the filamentous fungus Trichoderma reesei served as a representative source of enzymes for this study. Prior to chromatography, PAPC was tethered via its amino functional group to N-hydroxysuccinimide-activated agarose. The resulting affinity matrix specifically ...