Biological synthesis of isorhamnetin 3-O-glucoside using engineered glucosyltransferase

BG Kim, SH Sung, NR Jung, Y Chong…

Index: Kim, Bong-Gyu; Sung, Su Hyun; Jung, Na Ri; Chong, Youhoon; Ahn, Joong-Hoon Journal of Molecular Catalysis B: Enzymatic, 2010 , vol. 63, # 3-4 p. 194 - 199

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Citation Number: 7

Abstract

The gene for one of the glycosyltransferases from Populus deltoids, PGT-3, was cloned and was expressed as a glutathione S-transferase fusion protein in Escherichia coli. Various flavonoids were used as potential substrates of the purified recombinant PGT-3. Flavones having two adjacent hydroxyl groups were served as substrate. The regioselectivity of PGT-3 depends on the hydroxyl groups of the substrate. Flavones having two adjacent hydroxyl ...