Rational design, synthesis, evaluation and enzyme–substrate structures of improved fluorogenic substrates for family 6 glycoside hydrolases

…, T Ishida, H Hansson, M Sandgren, J Ståhlberg

Index: Wu, Miao; Nerinckx, Wim; Piens, Kathleen; Ishida, Takuya; Hansson, Henrik; Sandgren, Mats; Stahlberg, Jerry FEBS Journal, 2013 , vol. 280, # 1 p. 184 - 198

Full Text: HTML

Citation Number: 6

Abstract

Abstract Methylumbelliferyl-β–cellobioside (MUF–G2) is a convenient fluorogenic substrate for certain β–glycoside hydrolases (GH). However, hydrolysis of the aglycone is poor with GH family 6 enzymes (GH6), despite strong binding. Prediction of the orientation of the aglycone of MUF–G2 in the+ 1 subsite of Hypocrea jecorina Cel6A by automated docking suggested umbelliferyl modifications at C4 and C6 for improved recognition. Four ...