Determination of dehydroascorbic acid in mouse tissues and plasma by using tris (2-carboxyethyl) phosphine hydrochloride as reductant in metaphosphoric acid/ …
…, T Uchiki, M Iwama, Y Kishimoto, R Takahashi…
Index: Sato, Yasunori; Uchiki, Takayuki; Iwama, Mizuki; Kishimoto, Yuki; Takahashi, Ryoya; Ishigami, Akihito Biological and Pharmaceutical Bulletin, 2010 , vol. 33, # 3 p. 364 - 369
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Citation Number: 31
Abstract
Ascorbic acid (AA) has a strong anti-oxidant function evident as its ability to scavenge superoxide radicals in vitro. Moreover, AA is an essential ingredient for post-translational proline hydroxylation of collagen molecules. Dehydroascorbic acid (DHA), the oxidized form of AA, is generated from these reactions. In this study, we describe an improved method for assessing DHA in biological samples. The use of 35 mM tris (2-carboxyethyl) phosphine ...
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