Inactivation of Lactobacillus leichmannii ribonucleotide reductase by 2′, 2′-difluoro-2′-deoxycytidine 5′-triphosphate: Covalent modification

GJS Lohman, JA Stubbe

Index: Biochemistry, , vol. 49, # 7 p. 1404 - 1417

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Citation Number: 11

Abstract

... Biochemistry , 2010, 49 (7), pp 1404 ... The reaction mixture contained, in a final volume of 1 mL, 5 mM β-F 2 C, 10 mM ATP, 2 mM DTT, 0.5 mg/mL BSA, 1.4 mg/mL human dCK, 50 mM Tris (pH 7.6), 100 mM KCl, and 10 mM MgCl 2 . The reaction was initiated by addition of ...