Xenobiotica

Enzyme kinetics and substrate selectivities of rat glutathione S-transferase isoenzymes towards a series of new 2-substituted 1-chloro-4-nitrobenzenes

…, JNM Commandeur, JM Te Koppele, B Van Ommen…

Index: Van Der Aar; Buikema; Commandeur; Te Koppele; Van Ommen; Van Bladeren; Vermeulen Xenobiotica, 1996 , vol. 26, # 2 p. 143 - 155

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Citation Number: 16

Abstract

1. Four different rat glutathione S-transferase (GST) isoenzymes, belonging to three different classes, were examined for their GSH conjugating capacity towards 11 2-substituted 1- chloro-4-nitrobenzene derivatives. Significant differences were found in their enzyme kinetic parameters K m, k cat and k cat/K m. 2. Substrates with bulky substituents on the ortho- position appeared to have high affinities (low K m's) for the active site of the GST- ...