Synthesis of Oligonucleotides Containing 2′??Deoxyisoguanosine and 2′??Deoxy??5??methylisocytidine Using Phosphoramidite Chemistry

…, JD Rozzell, SA Benner, TR Battersby

Index: Jurczyk, Simona C.; Kodra, Janos T.; David Rozzell; Benner, Steven A.; Battersby, Thomas R. Helvetica Chimica Acta, 1998 , vol. 81, # 5 p. 793 - 811

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Citation Number: 32

Abstract

Abstract The synthesis of oligonucleotides containing 2′-deoxy-5-methylisocytidine and 2′-deoxyisoguanosine using phosphoramidite chemistry in solid-phase oligonucleotide synthesis is described. Supporting previous observations, the N, N-diisobutylformamidine moiety was found to be a far superior protecting group than N-benzoyl for 2′-deoxy-5- methylisocytidine. 2′-Deoxy-N 2-[(diisobutylamino) methylidene]-5′-(4, 4′- ...