Novel fluorescent ceramide derivatives for probing ceramidase substrate specificity

KP Bhabak, D Proksch, S Redmer, C Arenz

Index: Bhabak, Krishna P.; Proksch, Denny; Redmer, Susanne; Arenz, Christoph Bioorganic and Medicinal Chemistry, 2012 , vol. 20, # 20 p. 6154 - 6161

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Citation Number: 15

Abstract

Ceramidases are key regulators of cell fate. The biochemistry of different ceramidases and of their substrate ceramide appears to be complex, mainly due to specific biophysical characteristics at the water-membrane interface. In the present study, we describe the design and synthesis of a set of fluorescently labeled ceramides as substrates for acid and neutral ceramidases. For the first time we have replaced the commonly used polar NBD- ...