Intestinal absorption of fluorescence-derivatized cationic peptide 001-C8-NBD via adsorptive-mediated transcytosis

…, I Tamai, M Kamata, J Wakama, T Wakamiya…

Index: Sai, Yoshimichi; Kajita, Masahiro; Tamai, Ikumi; Kamata, Makoto; Wakama, Jun; Wakamiya, Tateaki; Tsuji, Akira Bioorganic and Medicinal Chemistry, 1998 , vol. 6, # 6 p. 841 - 848

Full Text: HTML

Citation Number: 21

Abstract

The intestinal absorption of an intact oligopeptide was investigated in rats using a synthetic cationic peptide, 001-C8 (H-MeTyr-Arg-MeArg-d-Leu-NH (CH2) 8NH2). The peptide was coupled with 4-nitrobenzo-2-oxa-1, 3-diazole (NBD) to prepare a fluorescence-labeled derivative 001-C8-NBD (H-MeTyr-Arg-MeArg-d-Leu-NH (CH2) 8NH-NBD) for the purpose of quantification. The degradation half-life of 001-C8-NBD in jejunal homogenate (1mg/mL) ...