Probing the mechanism of electron capture and electron transfer dissociation using tags with variable electron affinity

…, CK Chung, S Yin, P Ramachandran…

Index: Sohn, Chang Ho; Chung, Cheol K.; Yin, Sheng; Ramachandran, Prasanna; Loo, Joseph A.; Beauchamp, J. L. Journal of the American Chemical Society, 2009 , vol. 131, p. 5444 - 5459

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Citation Number: 78

Abstract

Electron capture dissociation (ECD) and electron transfer dissociation (ETD) of doubly protonated electron affinity (EA)-tuned peptides were studied to further illuminate the mechanism of these processes. The model peptide FQpSEEQQQTEDELQDK, containing a phosphoserine residue, was converted to EA-tuned peptides via β-elimination and Michael addition of various thiol compounds. These include propanyl, benzyl, 4-cyanobenzyl, ...