Synthesis of Oligonucleotide Derivatives with Aryl (trifluoromethyl) diazirine Moiety for the Photoaffinity Modification of Proteins and Nucleic Acids

YY Agapkina, DV Agapkin, AV Zagorodnikov…

Index: Agapkina; Agapkin; Zagorodnikov; Alekseev; Korshunova; Gottikh Russian Journal of Bioorganic Chemistry, 2002 , vol. 28, # 4 p. 293 - 299

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Citation Number: 2

Abstract

Abstract 1-(4-(3-(Trifluoromethyl)-3 H-diazirin-3-yl) benzamido)-3-O-(4, 4"-dimethoxytrityl)-2, 3-propanediol phosphoramidite was synthesized and used as a modified unit in the automatic synthesis of oligodeoxyribonucleotides. Pentadecathymidylates with various numbers of 2, 3-propanediol moieties substituted with aryl (trifluoromethyl) diazirinyl (ATFMD) were obtained, and the thermal stability of their duplexes with (dA) 15 were ...