Characterization of nicotinamidases: steady state kinetic parameters, classwide inhibition by nicotinaldehydes, and catalytic mechanism

JB French, Y Cen, TL Vrablik, P Xu, E Allen…

Index: Biochemistry, , vol. 49, # 49 p. 10421 - 10439

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Citation Number: 20

Abstract

... Biochemistry , 2010, 49 (49), pp 10421–10439. DOI ... A typical reaction mixture contained 1 mM α-ketoglutarate, 250 μM NAD(P)H, 1.5 units of GDH per 100 μL of reaction volume, and different concentrations of nicotinamide in 100 mM phosphate buffer (pH 7.3) at 25 °C. The ...