Synthesis of oligonucleotides containing site-specific carcinogen adducts. Preparation of the 2-cyanoethyl N, N-diisopropylphosphoramidite of N-(2'-deoxyguanosin-8- …

Y Zhou, S Chladek, LJ Romano

Index: Zhou, Yuanzhong; Chladek, Stanislav; Romano, Louis J. Journal of Organic Chemistry, 1994 , vol. 59, # 3 p. 556 - 563

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Citation Number: 22

Abstract

A 9-fluorenylmethoxycarbonyl (Fmoc) group was used to protect the exocyclic amine on the modified guanine of N-(2'-deoxyguanosin-8-yl)-2-(acetylamino) fluorene (dG-Ca-AAF) so that oligonucleotides containing a site-specific AAF adduct could be prepared. Reaction of Fmoc-C1 with dG-Ca-AAF gave